Stains and Dyes
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- (1)
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- (1)
- (1)
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- (366)
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- (1)
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- (1)
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- (1)
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- (1)
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- (1)
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- (1)
- (1)
- (1)
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- (1)
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- (147)
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- (2)
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- (45)
- (2)
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- (2)
- (2)
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- (57)
- (1)
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- (1)
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- (2)
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- (116)
- (2)
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- (1)
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- (2)
- (1)
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- (1)
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- (1)
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- (1)
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- (1)
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- (1)
- (2)
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- (2)
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- (1)
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- (12)
- (189)
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- (1)
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Biotium Calcein AM
Calcein AM is a widely used green fluorescent cell marker. Calcein AM is membrane-permeant and thus can be introduced into cells via incubation. Once inside the cells, calcein AM is hydrolyzed by endogenous esterase into the highly negatively charged green fluorescent calcein, which is retained in the cytoplasm. Calcein AM is an excellent tool for the studies of cell membrane integrity and for cell tracing. Supplied in lyophilized form or as a solution in anhydrous DMSO at 4 mM (80011-1) or 1 mg/mL (1 mM) (80011-2). Calcein AM is also offered in our Cell Viability and Cytotoxicity Assay Kit. Properties: Ex/Em (calcein) = 494/517 nm (pH 8); Colorless solid soluble in DMSO; Store at -20°C and protect from light; C46H46N2O23; MW: 995
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Biotium DII (DIIC18(3))
DiI (DiIC18(3); 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine) is a widely used carbocyanine membrane dye that labels cell membranes by inserting its two long (C18 carbon) hydrocarbon chains into the lipid bilayer. Particularly, it has been extensively used for the anterograde and retrograde labeling of neurons. The intense fluorescence and high photostability of the dye make it possible to visualize the fine structures (axons and dendrites) of the neurons. Due to its low toxicity and the tendency to give highly stable cell labeling, the dye has also been generally used for long term cell tracing of cells both in cultures and in living embryos or animals. Also offered as ready-to-use CellBrite™ Cytoplasmic Membrane Dyes. Properties: Ex/ Em(MeOH) = 549/565 nm; e = 148,000; Dark red solid soluble in ethanol, DMF or DMSO; Store at 4°C; C59H97ClN2O; MW: 933.88; [41085-99-8]
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Biotium RedDot™1, 200X in water
RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells. RedDot™1 is highly thermostable and photostable, providing convenient handling for demanding applications such as confocal microscopy. The stain may also be used for DNA content analysis by flow cytometry. The dye has an excitation/emission of 662/694 nm when bound to DNA. Properties: Liquid; Store at 4°C or -20°C, protect from light; Ex/Em= 662/694 nm (with DNA).
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Biotium Rhodamine 123
Rhodamine 123 is a popular green fluorescent mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. It is widely used in flow cytometry studies involving mitochondrial membrane potential. Please also see Dihydrorhodamine 123 and its HCl salt.Properties: Ex/Em(MeOH) = 505/534 nm; e (505 nm, MeOH) = 97,000; Orange red solid soluble in DMSO or DMF; Store at 4°C and protect from light, especially in solution; C21H17ClN2O3; MW: 381; [62669-70-9]
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Electron Microscopy Sciences UAR-EMS Uranyl Acetate Replacement 25 ML
A non-radioactive substitute for uranyl acetate with comparable results. Avoids the tedious authorization, shipping and disposal issues involving uranyl acetate without sacrificing performance. Two lanthanide salts, samarium and gadolinium triacetate, effectively used for staining plastic-embedded animal and plant tissues. Also for negative staining of macromolecules. Not recommended as a fixative.
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Scytek Laboratories Elastic Stain Kit (Modified Verhoff's): Small
The Elastic Stain Kit is intended for use in histological demonstration of elastin in tissue sections. Demonstration of elastic tissue is useful in cases of emphysema (atrophy of elastic tissue), arteriosclerosis (thinning and loss of elastic fibers) and various other vascular diseases.Elastic fibers: Black to Blue/BlackNuclei: Blue to BlackCollagen: RedMuscle & Other: YellowKit contents: HSV060, Hematoxylin Solution (5%), 60ml.FCC030, Ferric Chloride (10%, Aqueous), 30 ml.LIS030, Lugol’s Iodine Solution, 30 ml.FCB060, Ferric Chloride (2%) Differentiating Solution, 60 ml.STB030, Sodium Thiosulfate Solution (5%), 30 ml.VGS030, Van Gieson’s Solution, 30 ml.
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Biotium Thiazole Orange, 10mM in DMSO
Thiazole orange (TO) is a nucleic acid dye commonly used in reticulocyte analysis to stain residual RNA of blood cells, or to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange stains the yeast nucleus, and is also able to stain bacteria and mammalian cells. The dye has an excitation/emission of 512/533 nm when bound to DNA. Properties: Orange solution; Store dessicated at 4°C, protect from light; Ex/Em= 512/533 nm (with DNA); C26H24N2O3S2; MW: 476.61; [67-68-5].
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Biotium RH795
The styryl dye RH795 is a fast-responding potentiometric probe that is primarily used for functional imaging of neurons. The dye is spectrally similar to RH414, but exhibits different physiological effects during staining. For example, RH414 causes arterial constriction during cortex staining, while RH795 does not. The excitation/emission data below are for the dye in methanol. In cell membranes, the spectra of styryl dyes are typically blue-shifted by as much as 20 nm for absorption or excitation and 80 nm for emission. Properties: Ex/Em (MeOH) = 530/712 nm; Orange red solid soluble in H2O; Store at -20°C and protect from light; C26H39Br2N3O2; MW: 585.42; [172807-13-5]
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Biotium Sulforhodamine B
Like other sulforhodamine dyes, the highly water-soluble sulforhodamine B can be used as a polar tracer for cell morphology and neuronal cell-cell communication studies. Sulforhodamine B has also been shown to be a superior protein stain for quantification of cellular proteins in cultured cells. The dye is believed to bind to basic amino acids of proteins, and colorimetric measurement of protein-bound dye provides an estimate of the total protein mass as a measure of cell number. The assay is simple and reproducible, and the end-point measurement is not time-critical, a significant advantage over tetrazolium-based assays. The intensely colored, water-soluble dye is particularly useful for in vitro cell-based screening of anticancer drugs and well-suited to large scale screening of anticancer drug candidates from natural extracts or synthetic chemicals. Properties: Ex/Ex = 565/586 nm; Red solid soluble in water; Store at room temperature; C27H30N2O7S2; MW: 559; [2609-88-3]
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Biotium NucSpot Live 488, 1000X in DMSO
NucSpot Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have low toxicity for live cell imaging. The dyes are supplied as 1000X stock solutions in DMSO, and are supplied with a vial of verapamil, which may increase probe retention and staining in some cell types. NucSpot Live 488 stains nuclei with green fluorescence and has an excitation/emission of 500/515 nm. Properties: Ex/Em= 500/515 nm; Store at 4°C and protect from light.
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Biotium Thioflavin T "high purity grade"
A cell-permeable benzothiazole dye that exhibits enhanced fluorescence (Ex/Em 450/482 nm) upon binding to amyloid fibrils. Useful in monitoring stacked b-sheet aggregates, Thioflavin T has also been used in histology and for protein characterization.Biotium offers high purity grade of Thioflavin T with HPLC purity greater than 97%.Properties: Yellow solid soluble in DMF and DMSO; Stored desiccated at 4°C; C17H19ClN2S; MWt: 318.86; [2390-54-7]
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Electron Microscopy Sciences TFM™ Tissue Freezing Medium, Clear 4 OZ
An embedding matrix for frozen sections. TFM is an ultra pure formulation of water-soluble glycols and resins that provides a solid bond between the tissue and the object holder with the following features: less ice artifacts, less curling, and faster freezing.
Color: Clear
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GFS CHEMICALS INC STARCH INDICATOR SOL 4L
NC2962242 STARCH INDICATOR SOL 4L
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Electron Microscopy Sciences Giemsa Stain Solution 250 ML
Beck (1938); Pappanheim (1912). References Beck, R.C., Laboratory Manual of Hematological Technique, W.B. Saunders & Co., Philadelphia, c. 1938, pp 233 – 236
Clark, G. (ed), Staining Procedures, 3rd ed. Williams &Wilkins, Baltimore, c. 1973, pp 131 – 132
Strumia, M.M., J Lab Clin. Med 21:930 – 934, 1935 – 1936
Luna, L.G., (ed) Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology, 3rd ed. McGraw-Hill, NY, c. 1968, pp 121-122
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Electron Microscopy Sciences Mayers Hematoxylin 500 ML
Used in Maximow's Method for Bone Marrow, Morphology details of marrow cells. Mallory (1961). Morphology details of marrow cells. See AFIP Manual of Histologic Staining Methods, 3rd Ed, Ed. L. Luna: New York: McGraw Hill Publications, c. 1968, p. 120 or Mallory, F.B., Pathological technique, New York, Hafner Publishing Co., 1961, p.196
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